Macrophage harvesting, GIBCO buffer

Chance, Tim jtc3 at CDC.GOV
Thu Mar 21 07:40:34 EST 2002


Thanks to one of the list members, I made an error in my solution formula
for nonenzymatic disociation solution. It should be 0.01MPBS PH 7.5 and
0.60mM EDTA (NOT 60m EDTA.
thanks much Dee
Tim Chance
CDC

-----Original Message-----
From: Chance, Tim [mailto:jtc3 at CDC.GOV]
Sent: Tuesday, March 19, 2002 3:03 PM
To: cyto-inbox
Subject: RE: Macrophage harvesting, GIBCO buffer



The dissociation solution is expensive and you may obtain the same results
with o.01M PBS  with 60 mM EDTA. The EDTA is the active ingredient. It
chelates divalent cations that aid in attachment allowing the cells to break
loose without cellular damage.
Tim Chance

-----Original Message-----
From: Dee Harrison-Findik [mailto:duygu.harrison-findik at imvs.sa.gov.au]
Sent: Wednesday, March 13, 2002 6:30 PM
To: cyto-inbox
Subject: Macrophage harvesting, GIBCO buffer



Dear Flowers
Chinnaswamy has kindly forwarded the details of the cell dissociation buffer
from GIBCO to me as another alternative to harvest macrophages. His message
is below.
Cheers
Dee

-----Original Message-----
From: Chinnaswamy Jagannath [mailto:Chinnaswamy.Jagannath at uth.tmc.edu]
Sent: Thursday, 14 March 2002 12:11 am
To: cyto-inbox
Subject: GIBCO buffer


Ms. Dee: here are the specs for the GIBCO cell dissociation buffer; please
forward to appropriate people...

Cat NO: 13150-016
Component NO: 1096537
cell dis buf, enzyme free, HBSS based propritary formual for detaching cells
from plastic surfacces;cells can thne be tested for flow, ligand binding,
immunohstochemistry etc
Make: GIBCO BRL

I have tested such cells for mannose receptor and it works just fine; I
strongly recommend it for macrophage studies.

Dr.Jagannath



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