Vascular Smooth Muscle Cells
kenichi.fujise at uth.tmc.edu
Mon Mar 18 20:28:19 EST 2002
I am a cardiovascular investigator trying to isolate vascular smooth
muscle cells from rat carotid arteries at as high purity as possible.
Unfortunately, a previous attempt showed that only 25% of isolated
cells were alpha-actin positive after differentiation (heparin and 0.5%
sera for 4 days). Other cells appear to be endothelial cells.
Does anybody have a good protocol for vascular smooth muscle cell
In addition, do you have any cleaver way to use FACS to determine the
purity of the prep?
I thank you for your time and help on this.
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