injectable dye

Wayne Harris waharri at emory.edu
Thu Mar 14 13:34:38 EST 2002


Hello Flow-ers,

I  was hoping someone out there could lead me in the right direction. I was
interested in finding an injectable fluorescent dye (into mice) that would
hopefully label cells in a concentration dependent manner so that cells
local to the site of injection would be labelled more intensely than more
distant cells. My goal is to find a way to label resident cells and track
their movement. It doesn't have to be a long term label. I'm trying to look
at peritoneal exudate cells and what happens to them upon bacterial
infection in vivo. I'm completely lost on a dye that would label preferably
in something other than FL-1 and yet would not be too toxic to be used in
such a way. Maybe someone out there has a protocol for something like this.
I would deeply appreciate any help in this endeavour.

thanks


--
Wayne Harris
Lead Research Specialist

Department of Microbiology and Immunology
Emory University , School of Medicine







More information about the Cytometry mailing list