Macrophage harvesting, GIBCO buffer

Dee Harrison-Findik duygu.harrison-findik at imvs.sa.gov.au
Wed Mar 13 18:29:31 EST 2002


Dear Flowers
Chinnaswamy has kindly forwarded the details of the cell dissociation buffer
from GIBCO to me as another alternative to harvest macrophages. His message
is below.
Cheers
Dee

-----Original Message-----
From: Chinnaswamy Jagannath [mailto:Chinnaswamy.Jagannath at uth.tmc.edu]
Sent: Thursday, 14 March 2002 12:11 am
To: cyto-inbox
Subject: GIBCO buffer


Ms. Dee: here are the specs for the GIBCO cell dissociation buffer; please
forward to appropriate people...

Cat NO: 13150-016
Component NO: 1096537
cell dis buf, enzyme free, HBSS based propritary formual for detaching cells
from plastic surfacces;cells can thne be tested for flow, ligand binding,
immunohstochemistry etc
Make: GIBCO BRL

I have tested such cells for mannose receptor and it works just fine; I
strongly recommend it for macrophage studies.

Dr.Jagannath





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