GFP in U937?

Andrew Beernink ABeernink at novasite.com
Wed Mar 13 13:55:58 EST 2002


Greetings, guys & gals!

This is a question only peripherally related to flow (the answer will be
DIRECTLY related!):

Does anybody have any experience transfecting Clontech's fluorescent
proteins into U937 cells?  We've tried electroporation and lipid-based
reagents, with pathetic results.  The same vectors happily transfected into
HEK293 cells, so I'm convinced the vector is good.

Thanks for any suggestions.

Andrew A. Beernink
Research Scientist
Manager, Flow Cytometry
Novasite Pharmaceuticals, Inc.
11095 Flintkote
San Diego, CA  92121
858) 638-8584




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