Lysis of blood cells?

Stankovic, Ana avs2 at CDC.GOV
Tue Mar 12 11:45:12 EST 2002


I would not mix blood groups if possible. It is less likely that you will
get hemolysis, but you will definitely get red cell aggregation
(agglutination). The only antibodies that you should concern yourself with
are the "naturally" occurring anti-A and anti-B red cell antibodies.
Antibodies against most other blood groups do not occur as frequently (only
as a result of transfusion or pregnancy), so they should not be of major
concern. Regarding leukocytes it is possible that anti-HLA antibodies in
multiparous women can cause leukocyte lysis, activation and aggregation.
However, if you have to mix blood try to mix the same ABO types.

Ana K. Stankovic, MD, PhD
Centers for Disease Control and Prevention
Public Health Practice Program Office
Division of Laboratory Systems
Laboratory Practice Assessment Branch
4770 Buford Highway
MS G23
Atlanta, GA 30341
phone: (770) 488-8131
fax: (770) 488-8275

-----Original Message-----
From: MOVERARE, ROBERT [GSO/0454] [mailto:robert.moverare at]
Sent: Monday, March 11, 2002 5:29 AM
To: cyto-inbox
Subject: Lysis of blood cells?

Dear Flowers,

I have a rather basic question. Is it possible to mix blood samples from
different donors without the problem of lysis of the blood cells due to
complement-mediated cytolysis, i.e. if the donors blood groups are
miss-matched? For example, if blood from an "AB, Rh+ donor" is mixed with
blood from an "O, Rh- donor" and then left on the bench for some hours at
room temperature for later analysis by flow cytometry. Could it be harmful
for the cells (erythrocytes or leucocytes)?

Thanks in advance,


Robert Movérare, Ph.D.
Pharmacia Diagnostics AB
SE-751 82 Uppsala

e-mail: robert.moverare at

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