UV excitable dyes for surface phenotyping - ELF-97?

Adrian Smith A.Smith at centenary.usyd.edu.AU
Tue Mar 12 20:31:23 EST 2002


Hi all,
	We are in the process of getting some extra colours working
on our new FACSVantage SE and are trying to find a dye we can excite
with the third laser (a mixed gas argon and krypton (Coherent Innova
70 Spectrum UV)).

I have read the Molecular Probes literatures and the publication by
Telford et al (Cytometry 32:117-125 (2001)) about the use of the
ELF-97 enzyme substrate system and was wondering if anyone has any
"real-world" experience using it with surface antigens? I am
particularly concerned about the 30min incubation at RT that is
required. What does this do to live cells? (the MP literature
suggests using fixed cells but the Cytometry paper seems to use live
cells). What about the labelling of other surface markers?

Any other suggestions for alternative dyes that we can use with this laser?

Thanks,

Adrian Smith

--
______________________________________________
Adrian Smith (Research Officer)        T CELL BIOLOGY GROUP
Centenary Institute of Cancer Medicine & Cell Biology
Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA.
Ph: (02) 9565-6198 Fax: (02)-9565-6103



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