anti-STAT staining

Andrew D. Wells adwells at mail.MED.UPENN.EDU
Tue Mar 12 08:53:12 EST 2002


We were able to detect phospho-STAT5 quite nicely by flow cytometry in
murine T cells stimulated with IL-2 using Zymed¹s anti-phospho-STAT5 reagent
and a formaldehyde/methanol fixation procedure.  The method and data are
published in the Journal of Immunology 165:2432, 2000.  I think the company
claims reactivity to both mouse and human, but we¹ve only tried mouse.  Most
of these phospho-acceptor domains are conserved between mouse and human.
Let me know if you need further details.



Andrew D. Wells, Ph.D.
Assistant Professor
Department of Pathology and Laboratory Medicine
University of Pennsylvania
Biesecker Liver Disease Center
Children's Hospital of Philadelphia
807C Abramson Research Center
34th and Civic Center Boulevard
Philadelphia, PA  19104

phone: (215) 590-8710
fax:   (215) 590-7384 

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