anti-STAT staining

Calman Prussin CPRUSSIN at
Fri Mar 8 11:41:26 EST 2002

Tom Fleisher's groups has reported flow cytometric detection of
phosphorylated STAT-4 by using an anti-phospho STAT-4 antibody and
intracellular staining (Uzel, Clin Imm, 100, pg. 270, 2001).

I am trying to stain for phospho-STAT-5. Does anyone have any experience
with this? I would be grateful to run a few questions by you in relation to
choice of antibody, etc. If your help makes a substantial contribution to
the resultant paper I would be happy to make you a co-author. thanks.

> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health
> Building 10, Room 11C205, MSC-1881
> Bethesda, MD	20892-1881
> (301) 496-1306 (Voice)
> (301) 496-1306 (Fax)
> calman at
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