SP with LSR

Houston, Jim Jim.Houston at stjude.org
Tue Mar 5 09:43:08 EST 2002

If you co-layer the UV and the HeNe in the 3rd position you can detect the
red signal from the HO dye in FL3.  My LSR is configured with a change in
laser alignment and pinholes to allow UV/HeNe laser to be seen in FL3.  Not
so hard to do.

Contact  BD if unable to re-align yourself.

Jim Houston
> ----------
> From:		David Dombkowski
> Sent:		Monday, March 4, 2002 7:32 AM
> To:	Cytometry Mailing List
> Subject:	SP with LSR
> Kimmo,
>     You will not see red fluorescence with Hoechst 33342 with the
> configuration of 2 PMT UV, 3 or 4 PMT Argon and 1 PMT He-Ne. The red
> fluorescence is too high for dichroic to reflect to PMT for UV. You need
> to
> be able to collect this signal in PMT usually dedicated to Argon or He-Ne
> laser. There is no pinhole for UV fluorescence in these channels. You need
> a custon configuration which may be achieved if you cantact Becton
> Dickinson.
> David
> David M. Dombkowski
> dombkowski at helix.mgh.harvard.edu
> Flow Cytometry-Pathology-CNY rm7017
> 149 13th Street
> Massachusetts General Hospital-East
> Charlestown, MA 02129
> Tel. (617)726-1683
> Fax.(617)724-3164

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