detecting autoantiboy on cell surface (human)

Zand, Martin Martin_Zand at URMC.Rochester.edu
Mon Mar 4 08:08:56 EST 2002


Yes.  We detect alloantibody on the surface of human leukocytes in organ
transplantation routinely.  Jackson Immunolabs sells Fab2 anti-human Ig(H+L)
for IgM and IgG conjugated to various fluorochromes.  You will want to
co-stain with anti-CD3 to exclude staining of B cells, which can be
problematic (express FcR, and surface Ig)

Martin S. Zand, MD, PhD
Assistant Professor of Medicine and Medical Humanities
Medical Director, Kidney and Pancreas Transplant Programs
University of Rochester Medical Center
601 Elmwood Ave - Box 675
Rochester, NY 14642

Vox:  585.275.4517
Fax:  585.442.9201


-----Original Message-----
From: Fang-yao Stephen HOU [mailto:shou at uci.edu]
Sent: Saturday, March 02, 2002 5:24 AM
To: cyto-inbox
Subject: detecting autoantiboy on cell surface (human)



Dear experts in clinical cytometry:

One of my users asks me if it is feasible to detect human IgM/IgG on
surface of leukocytes. The white count of the patient is between 30k -
40k. The oncologist is looking for venders with supply of anti-human IgM &
IgG, conjugated with fluorochromes. A related literature regarding the
detection of surface bound autoantibody will be very helpful as well.
Thank you very much.

Fang-Yao Stephen Hou
Flow Cytometry Specialist
UC-Irvine



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