detecting autoantiboy on cell surface (human)

Paula Fukushima pif at mail.nih.gov
Mon Mar 4 09:26:14 EST 2002


Hi,
    We do this routinely. The specimen needs to be washed at least three
times with PBS. We use anti-IgM, IgD, IgG and IgA(all in FITC) from
Biosource International, Burlinggame, CA.  These need to be paired with a B
cell marker. We routinely pair them with CD19 or CD20, depending on what
therapy the patients are getting. In difficult to interpret cases, use the
light chain reagents to compare.


                 Paula
  At 02:24 AM 3/2/2002 -0800, Fang-yao Stephen HOU wrote:

>Dear experts in clinical cytometry:
>
>One of my users asks me if it is feasible to detect human IgM/IgG on
>surface of leukocytes. The white count of the patient is between 30k -
>40k. The oncologist is looking for venders with supply of anti-human IgM &
>IgG, conjugated with fluorochromes. A related literature regarding the
>detection of surface bound autoantibody will be very helpful as well.
>Thank you very much.
>
>Fang-Yao Stephen Hou
>Flow Cytometry Specialist
>UC-Irvine



Paula Fukushima
Flow Cytometry
LP, NCI, NIH
10 Center Drive MSC-1500
Bethesda, Md. 20892-1500






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