Please help: which anti-CD10 clones work well on lymph nodes ?

Jáksó Pál Pal.Jakso at aok.pte.hu
Fri Jan 25 02:44:21 EST 2002


Dear flowers,

One of our pathologists complained about our CD10 flow results of lymph
node biopsies and fine needle aspirates, because they don't match with
immuohistochemical staining which shows positivity of lymph nodes
germinal center cells and follicular lymphoma while the flow shows
negativity or very dim stain although this antibody works well in B-cell
acut lymphoid leikaemia. Currently we use DAKO (clone: SS2/36) for flow
and Novocastra CD10 (clone: 56C6) for immunohistochemistry. I think  the
different clones may give different staining.
Novocastra does not produce fluorescent labelled CD10 for flow and I
have to look for other sources to try different CD10 clones wich give
comparable result with the immunohistochemistry in the case of lymph
nodes.
Has anybody experience on this ?

Thanks,

Pal Jakso

University of Pecs
Faculty of Medicine
Dept. of Pathology
7643, Hungary
12. Szigeti str.




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