UV lasers

Frederic Preffer preffer at helix.mgh.harvard.edu
Tue Feb 5 20:30:03 EST 2002


Dear Richard

I notice Howard has provided you with an exhaustive choice of
possibilities. While all excellent suggestions, the first thing i would
recommend is that you call or email phil fostini at Evergreen Lasers  at
860 349-1797 or  support at evergreenlaser.com.  Phil might be able to help
you accurately diagnose the situation and perhaps keep your laser alive for
as long as possible in its present state. If you need to replace the laser
[and as howard indicates, your water and electric bills   factor favorably
into your cost/benefit budget analysis], Evergreen can retube the laser at
a phenominal savings.

F Preffer



At 02:51 PM 2/1/2002 , Richard K. Meister wrote:
>
>Hi, everyone:
>
>I'm running an EPICS Elite with a gated amplifier and 5 PMTs.  We have 3
>lasers, one of which is an old Coherent Innova 90 which we have been using
>for its UV wavelengths.  Unfortunately, it has been running "over pressure"
>for some time, and now gives me an "under voltage" error message.  It now
>will not operate in "light regulation" mode, so I think that it's
>usefullness as a UV source is at an end.  So, what to do now (with a
>limited budget)?
>
>I've heard that there are air-cooled UV lasers now available.  Do any of
>you have experience using one of these for flow?  Are they adequate for
>doing calcium studies with indo-1 or DNA with Hoechst 33342?  What
>manufacture/model do you recommend?
>
>Or, are water-cooled lasers still preferred for flow work?  If so, please
>give me your recommendation for getting the plasma tube replaced in our old
>Innova 90.  (Or is there some reason not to try to repair the Innova 90?)
>
>Thanks in advance for your advice.
>
>Rick Meister
>* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
>*  Richard K. Meister                  Email:  meister.1 at osu.edu  *
>*  The Ohio State University           Voice:  (614) 292-9716     *
>*  Dept. of Veterinary Biosciences     FAX:    (614) 292-6473     *
>*  Cytometry Instrumentation Lab                                  *
>*  1925 Coffey Road                                               *
>*  Columbus, OH  43210  U.S.A.                                    *
>* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
>


Frederic I. Preffer
Department of Pathology
Charlestown Navy Yard- 7140
149 13th Street
Massachusetts General Hospital -East
Charlestown, MA 02129

voice [617] 726-7481
   fax [617] 724-3164




More information about the Cytometry mailing list