Antibody Conjugation Vendor

Hyun, Bill hyun at cc.ucsf.edu
Tue Feb 5 17:25:37 EST 2002


Dick,
Could you name some dyes after my friends?

Dave "Background" Coder
Howard "Quantum" Shapiro
Paul "Quench" Robinson

Thanks,
-Billy Bleach



-----Original Message-----
From: Richard Haugland [mailto:richard.haugland at probes.com]
Sent: Monday, February 04, 2002 3:54 PM
To: cyto-inbox
Subject: Re: Antibody Conjugation Vendor



Alexa (Fluor) dyes are named after my son Alexander (feminized a bit,
however).

Marina Blue dyes are named after my daughter Marina.

Dick Haugland



DAVID M CODER wrote:

> Jim,
>
> A very effective do-it-yourself approach is the one of the Alexa Fluor
dyes
> from Molecular Probes. You can select the dye that is most compatible with
> your red laser (HeNe to Kr) and filters. Conjugations are very easy to do
> and give stable products. We've found that even the Alexa Fluor 680 is
> excited sufficiently well with a 17mW HeNe laser at 633nm on the LSR to
give
> a good signal with highly expressed surface antigens.
>
> (And one wonders: Who is Alexa, anyway?)
>
> Dave
> ----------------
> David M. Coder, Ph.D.
> Consultant in Cytometry
> email: d_coder at msn.com
> tel./messages: 206-499-3446
>
> ----- Original Message -----
> From: "Houston, Jim " <Jim.Houston at stjude.org>
> To: cyto-inbox
> Sent: Thursday, January 31, 2002 12:53 PM
> Subject: Antibody Conjugation Vendor
>
> >
> >
> > To All:
> >
> > I am in need of getting two monoclonal antibodies conjugated to APC.
> > If you have had this done by some company/lab and liked the result
> > would you please let me know.
> >
> > thanks
> >
> > Jim Houston
> >



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