Antibody Conjugation Vendor
hyun at cc.ucsf.edu
Tue Feb 5 17:25:37 EST 2002
Could you name some dyes after my friends?
Dave "Background" Coder
Howard "Quantum" Shapiro
Paul "Quench" Robinson
From: Richard Haugland [mailto:richard.haugland at probes.com]
Sent: Monday, February 04, 2002 3:54 PM
Subject: Re: Antibody Conjugation Vendor
Alexa (Fluor) dyes are named after my son Alexander (feminized a bit,
Marina Blue dyes are named after my daughter Marina.
DAVID M CODER wrote:
> A very effective do-it-yourself approach is the one of the Alexa Fluor
> from Molecular Probes. You can select the dye that is most compatible with
> your red laser (HeNe to Kr) and filters. Conjugations are very easy to do
> and give stable products. We've found that even the Alexa Fluor 680 is
> excited sufficiently well with a 17mW HeNe laser at 633nm on the LSR to
> a good signal with highly expressed surface antigens.
> (And one wonders: Who is Alexa, anyway?)
> David M. Coder, Ph.D.
> Consultant in Cytometry
> email: d_coder at msn.com
> tel./messages: 206-499-3446
> ----- Original Message -----
> From: "Houston, Jim " <Jim.Houston at stjude.org>
> To: cyto-inbox
> Sent: Thursday, January 31, 2002 12:53 PM
> Subject: Antibody Conjugation Vendor
> > To All:
> > I am in need of getting two monoclonal antibodies conjugated to APC.
> > If you have had this done by some company/lab and liked the result
> > would you please let me know.
> > thanks
> > Jim Houston
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