Cardiomyocytes and flow

Bruce Milthorpe brucem at
Wed Feb 6 20:19:28 EST 2002

Dear cytometrists,
I have a colleague who would like to look at DNA profiles of foetal
cardiomyocytes by flow (MoFlo) using PI or EB. Andreas Simm had some
problems a few years back with PI for the cardiomyocytes and I wondered
if that had been solved satisfactorily whilst still allowing the cells
to be kept intact for other markers?  Also are there any protocols
specifying the general flow conditions (nozzle size etc)?

Many thanks,
Bruce Milthorpe

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