help with dotplots

Howard Shapiro hms at shapirolab.com
Mon Dec 23 15:08:42 EST 2002


Rachel Gerstein wrote-

>use contour plots, but note the number of cells displayed in the plot if
>its a small
>number

In response to Niga Nawroly's posting:

> > One of our lab's searchers have been looking at gamma delta T cells (very
> > low percentage of PBMCs).  Now, he wants to present his data in dotplots
> > (paper quality). The problem is that, he did not acquire enough cells to be
> > able to see a nice population when it is printed out.
> >
> > My question is to know if there is a way by using a software to make the
> > dots look more realistic (each dot representing one cell or half a cell!).
> >
> > PS: We use winlist, cellquest and WinMDI
> >

In this instance, contour plots will make the data look prettier, not more
realistic. You can't get more realistic than the dot plot. I think it's
fair to say that some of the most vocal advocates of contour plots favor
overlaying the single occurrences as dots when it is these low-frequency
events which are of significance. But, whether you do the contour plot, the
contour plot with overlaid dots, or the dot plot, you should note the
number(s) of cells represented.

-Howard





More information about the Cytometry mailing list