RBC label

Dr. John Waitumbi JWaitumbi at kisian.mimcom.net
Thu Aug 15 01:16:49 EST 2002


Dear Rick,
Try using PKH-26 (Sigma) to label the RBCs (you can purify them before
putting the label). The transfer of the dye to non labeled cells is minimal.

John

----- Original Message -----
From: "Richard K. Meister" <meister.1 at osu.edu>
To: cyto-inbox
Sent: Tuesday, August 13, 2002 7:28 PM
Subject: RBC label


>
> Hello, everyone!
>
> I have a colleague who wants to draw blood from a dog, label the
> erythrocytes with a permanent (or semi-permanent) fluorescent tag,
reinfuse
> the cells into the dog and later (15 minutes) draw blood from the dog
again
> and run the RBCs on the flow cytometer to determine the recovery of
> previously-labeled cells.  The proposal sounded simple enough to me until
I
> started to look for the required stain or monoclonal antibody that would
> specifically and uniquely stain the RBCs in a sample of whole blood.
>
> I haven't found anything yet, so any ideas you might have would be
welcomed.
>
> Thanks in advance.
>
> Rick Meister
>
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> *  Richard K. Meister                  Email:  meister.1 at osu.edu  *
> *  The Ohio State University           Voice:  (614) 292-9716     *
> *  Dept. of Veterinary Biosciences     FAX:    (614) 292-6473     *
> *  Cytometry Instrumentation Lab                                  *
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