gfp spectral shifts?
lwarma at med.unc.edu
Tue Aug 13 15:37:12 EST 2002
I'm not sure about this. What I do know is that DsRed1 takes about 48hrs
for the protein to mature to get full intensity but I'm not aware of
spectral shift during this time although it might be true. Try the
ClonTech site and look at DsRed2 which discusses the maturation issues
since these are at least partly solved with DsRed2.
At 07:49 AM 8/13/2002 -0700, you wrote:
>>Perhaps another (highly intersting) shift-inducing parameter exists
>>: protein maturation. It is known that newly produced dsRed, a
>>distant GFP homolog protein has a "greener" emission spectrum than
>>the mature dsRed-protein. This might be the case for GFP as well ...
>Interesting! Do you have a reference on this? We have used dsRed here
>only a little so haven't seen this behavior.
>>Marty Bigos wrote:
>>>Does anyone know of shifts in the emission spectra of the various gfp
>>>constructs due to intracellular pH, linkage to other proteins, etc.?
>>>Director, Flow Core
>>>Gladstone Institute of Virology and Immunology
>>>Building 3 SFGH Rm 509
>>Jan Bayer, Ph.D.
>>Fondation Jean Dausset - CEPH Email : bayer at cephb.fr
>>27, Rue Juliette Dodu Tel : +33 1 53 72 51 14
>>75010 Paris, France Fax : +33 1 53 72 51 28
Larry W. Arnold, Ph.D.
Director, Flow Cytometry Facility
Department of Microbiology and Immunology
Lineberger Comprehensive Cancer Center
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
More information about the Cytometry