AB's for human perforin, granzymes
MPAVLICK at niaid.nih.gov
Mon Aug 12 11:16:56 EST 2002
Very kind of you to write. I do appreciate your sending me this
I'm encouraged by all of the responses I've gotten, and will try to
go forward with this.
> From: Ondrej Hrusak
> Sent: Monday, August 12, 2002 3:48 AM
> To: Mark Pavlick; Cytometry Mailing List
> Subject: Re: AB's for human perforin, granzymes
> PE labelled Anti-perforin mAb from Pharmingen (BD) cat 556437 works OK for
> us in NK cells. We are currently working on the CD8+ T cells that seem to
> be more problematic.
> K. Kogawa et al. used it for both NK and CTL with an apparent success:
> Good luck,
> Ondrej Hrusak
> . . . . . . . . . . . . . . . . . . . . . . . . . .
> Ondrej Hrusak, M.D., Ph.D.
> Institute of Immunology
> Ondrej.Hrusak at lfmotol.cuni.cz
> fax +4202 2443 5962
> phone +4202 2443 5969, +4202 2443 2084
> postal address: V uvalu 84
> 150 06 Praha 5
> Czech Republic
> At 21:21 -0400 8.8.2002, Mark Pavlick wrote:
> List members,
> I want to stain human CD8 lymphocytes for the expression of
> perforin and granzymes A and B. I'd appreciate any references to currently
> available antibodies for this purpose. Thanks in advance.
> Mark Pavlick
> mvp at nih.gov
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