AB's for human perforin, granzymes

Mark Pavlick MPAVLICK at niaid.nih.gov
Mon Aug 12 11:16:56 EST 2002


Dr. Hrusak,

	Very kind of you to write. I do appreciate your sending me this
info.
	I'm encouraged by all of the responses I've gotten, and will try to
go forward with this.


Mark Pavlick

> ----------
> From:		Ondrej Hrusak
> Sent:		Monday, August 12, 2002 3:48 AM
> To:	Mark Pavlick; Cytometry Mailing List
> Subject:	Re: AB's for human perforin, granzymes
>
> Hello,
> PE labelled Anti-perforin mAb from Pharmingen (BD) cat 556437 works OK for
> us in NK cells. We are currently working on the CD8+ T cells that seem to
> be more problematic.
> K. Kogawa et al. used it for both NK and CTL with an apparent success:
> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=retrieve&db=pubmed&list_
> uids=11756153&dopt=Abstract
>
> Good luck,
> Ondrej Hrusak
>
>
> . . . . . . . . . . . . . . . . . . . . . . . . . .
> Ondrej Hrusak, M.D., Ph.D.
> Institute of Immunology
> Ondrej.Hrusak at lfmotol.cuni.cz
> fax +4202 2443 5962
> http://www.lf2.cuni.cz/clip/
> phone +4202 2443 5969, +4202 2443 2084
> postal address:      V uvalu 84
>			150 06 Praha 5
>			  Czech Republic
>
> At 21:21 -0400 8.8.2002, Mark Pavlick wrote:
>
>
> 8/8
>	List members,
>
>
>		I want to stain human CD8 lymphocytes for the expression of
> perforin and granzymes A and B. I'd appreciate any references to currently
> available antibodies for this purpose. Thanks in advance.
>
>
>
> Mark Pavlick
>
> mvp at nih.gov
>
>
>
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