cd4 cd8 coexpression

Howard.Gale@med.va.gov Howard.Gale at med.va.gov
Fri Nov 2 08:12:34 EST 2001


CD4+/CD8+/CD3+ cells are 1-4% range of the lymphocyte population from our
HIV+ patients. Most of the cells are brightCD4+/dimCD8+ but all combinations
of bright and dim are possible. We include these dual positive cells in both
the CD3+/CD4+ and CD3+/CD8+ counts. What do other labs do with these cells
and why?

-----Original Message-----
From: Kenneth Ault [mailto:aultk at mmc.org]
Sent: Wednesday, October 31, 2001 7:32 PM
To: cyto-inbox
Subject: Re: cd4 cd8 coexpression



A phenomenon frequently forgotten is coincidence.  If two cells enter the
observation
volume at the same time they will be seen as one event with the properties
of both cells.
This is a very common problem in my world (platelets) and should be
considered as a
possible explanation for any kind of unexpected dual expression of markers.
It would
be interesting to know if the frequency of CD4/CD8 doubles changes as the
particle
flow rate changes (i.e change the sample pressure or dilute the specimen.)

Ken Ault



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