Hi FLOWers,

Simon Monard smonard at trudeauinstitute.org
Mon Mar 12 16:18:21 EST 2001


Hi

People always used to incubate on ice for 30-60 minutes to try to prevent capping, also
to create a convenient time to have lunch or extended coffee and smoking breaks. Nowdays
you can add a tad of Sodium azide to prevent capping.

Simon

Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352


>>> "Heather Medbury" <Heather_Medbury at wsahs.nsw.gov.au> - 3/11/2001 5:50 PM >>>

Hi FLOWers,

About 5 years ago when I was analysing lymphocytes, we used to have the cell preparations
at 4 C for labelling with the antibody.
The work I do now on whole blood, the cells are labelled at room temperature.

Why do people label at 4 C when it works perfectly well at room temp?

Heather



Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
--------------------------------------------------------------
     All experiments are preliminary

------------------------o0)(0o--------------------------------------

  "In his heart a man plans his course
    but the Lord determines his steps"

---------------------------------------------------------------





More information about the Cytometry mailing list