viability gating

MSchwa3722@AOL.COM MSchwa3722 at AOL.COM
Sun Mar 4 18:36:53 EST 2001


I'm thinking of trying DAPI in the UV channel for live/dead gating instead of
using 7-AAD in FL3, in order to free FL3 for three color gating and sorting
strategies, on our FACS Vantage.  My question is:  Is there any spectral
overlap/interference with the standard FL1, FL2 or FL3, ie. FITC, PE and
PE-Cy5 or any other considerations I should be thinking of to do live/dead
gating with DAPI?

Your assistance, as always, is appreciated.

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