SYTO red and HOECHST or DAPI

Geoffrey Osborne geoff.osborne at anu.edu.au
Fri Jun 8 09:58:20 EST 2001


Elena,
	I think a paper worth looking at, which is relevant to your question, is
K.Toba et al./Journal of Immunological methods 182 (1995) 193-207.
	They compared five "DNA/RNA" dyes (PI,7AAD,AO,PY,TO) and for stated
voltages and compensation settings on a particular instrument provide a
table for comparison of emission spectra and nucleic acid binding
characteristics.
	Hope this helps
Geoff
At 12:20 PM 6/6/01 +0200, you wrote:
>
>My intention is to use SYTO red for RNA specific staining like I use
>Pyronine- Y. Does anyone knows if this possible?
>
>When I stain the DNA with Hoechst or DAPI (selective for DNA), the
>Pyronine-Y finds no place in these molecules, and therefore binds only to
>the RNA, wich is then the only nucleic acid that has free binding points.
>
>-> Could I block the DNA with Hoechst or DAPI and then stain with SYTO red
>to detect the RNA?
>
>Would SYTO red still find free binding points when the DNA is allready
>stained with one of these DNA selective fluorochromes?
>
>Thanks for your attention!
>
>Elena.
>
>
>
>
>-----------------------------------------
>Elena Soriano
>c/o Institut für Angewandte Mikrobiologie
>    Univ.f.Bodenkultur Wien
>
>Tel: +43 1 36006 6241
>Fax: +43 1 36 97 615
>http://www.boku.ac.at/iam

Geoffrey Osborne

Specialist, Flow Cytometry,
John Curtin School of Medical Research,
The Australian National University,
Canberra, 0200, ACT. AUSTRALIA
email: geoff.osborne at anu.edu.au
http://jcsmr.anu.edu.au/facslab/facshome.html

(61 2) 6125 3694.



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