FACScalibur instrument settings for PI ploidy/apoptosis acquisition
simmmmer at yahoo.com
Mon Jul 30 14:51:46 EST 2001
I'm posting this on behalf of a collegue. She's doing an assay with
just PI looking at the peaks before/after incubation of her cells in
pro-apoptotic drugs. If anyone looks at PI on a Calibur, could you
fill me in on the instrument settings that work the best? I would
appreciate any other tips.
>find out voltage settings for the PI assay. in particular ask about
>a printout of an acquisition template they use. also -
>fsc lin or log? what voltage?
>ssc same as above
>what about compensation?
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