Can EYFP be run on FACScan?

David Coder dcoder1 at qwest.net
Tue Jul 24 10:08:11 EST 2001


Dear Nan,
The absorption of YFP is about half at 488nm, so you should see some signal
though it may be dim depending on the level of expression; the FL1 channel
will be the best choice for detection. You can see the details at:
http://nucleus.immunol.washington.edu/Research_facilities/Apps/gfp_overview.
html

The web site that I built is still there even though I have been an
independent consult in cytometry since 1 June of this year.

Dave
----------------
David M. Coder, Ph.D.
email: dcoder1 at qwest.net
tel./messages: 206-499-3446

----- Original Message -----
From: "Nancy Harvey" <nharvey at microbio.umsmed.edu>
To: cyto-inbox
Sent: Monday, July 23, 2001 9:00 AM
Subject: Can EYFP be run on FACScan?


>
> Hello,
> I have an investigator who wants to check samples that have enhanced YFP.
The ideal
> excitation is 514.  Has anyone been able to detect EYFP with the 488
excitation of
> a FACScan?
>
> I could tune the primary laser on the Vantage to 514, but have only 488/10
filters
> for FSC and SSC.
>
> Any thoughts and suggestions are appreciated.
>
> Nan Harvey
> University of MS Medical Center
> Jackson, MS  39216
>
>




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