Can EYFP be run on FACScan?

Gerstein, Rachel Rachel.Gerstein at umassmed.edu
Tue Jul 24 09:47:07 EST 2001


YFP is readily detectable using 488 ex. and FL1 on the FACScan

=======================================================
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)


> ----------
> From:		Nancy Harvey
> Sent:		Monday, July 23, 2001 12:00 PM
> To:	Cytometry Mailing List
> Subject:	Can EYFP be run on FACScan?
>
>
> Hello,
> I have an investigator who wants to check samples that have enhanced YFP.
> The ideal
> excitation is 514.  Has anyone been able to detect EYFP with the 488
> excitation of
> a FACScan?
>
> I could tune the primary laser on the Vantage to 514, but have only 488/10
> filters
> for FSC and SSC.
>
> Any thoughts and suggestions are appreciated.
>
> Nan Harvey
> University of MS Medical Center
> Jackson, MS  39216
>
>



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