Can EYFP be run on FACScan?
Rachel.Gerstein at umassmed.edu
Tue Jul 24 09:47:07 EST 2001
YFP is readily detectable using 488 ex. and FL1 on the FACScan
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-5920 (FAX)
> From: Nancy Harvey
> Sent: Monday, July 23, 2001 12:00 PM
> To: Cytometry Mailing List
> Subject: Can EYFP be run on FACScan?
> I have an investigator who wants to check samples that have enhanced YFP.
> The ideal
> excitation is 514. Has anyone been able to detect EYFP with the 488
> excitation of
> a FACScan?
> I could tune the primary laser on the Vantage to 514, but have only 488/10
> for FSC and SSC.
> Any thoughts and suggestions are appreciated.
> Nan Harvey
> University of MS Medical Center
> Jackson, MS 39216
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