Can EYFP be run on FACScan?

Mark Kukuruga kukuru at med.umich.edu
Tue Jul 24 08:42:29 EST 2001


Nancy,
We've done EYFP on our Elite sorter using an air-cooled argon 488 nm line, about 15 mW.
So, I would suspect this would also work on your Scan.
MAK.

--
Mark A. KuKuruga, Managing Director
University of Michigan Flow Core
7416 CCGC 0946
(734) 647-3216, fax (734) 936-7376
kukuru at umich.edu


>>> "Nancy Harvey" <nharvey at microbio.umsmed.edu> 07/23/01 12:00PM >>>

Hello,
I have an investigator who wants to check samples that have enhanced YFP.  The ideal
excitation is 514.  Has anyone been able to detect EYFP with the 488 excitation of
a FACScan?

I could tune the primary laser on the Vantage to 514, but have only 488/10 filters
for FSC and SSC.

Any thoughts and suggestions are appreciated.

Nan Harvey
University of MS Medical Center
Jackson, MS  39216





More information about the Cytometry mailing list