Can EYFP be run on FACScan?

Nancy Harvey nharvey at microbio.umsmed.edu
Mon Jul 23 11:00:37 EST 2001


Hello,
I have an investigator who wants to check samples that have enhanced YFP.  The ideal
excitation is 514.  Has anyone been able to detect EYFP with the 488 excitation of
a FACScan?

I could tune the primary laser on the Vantage to 514, but have only 488/10 filters
for FSC and SSC.

Any thoughts and suggestions are appreciated.

Nan Harvey
University of MS Medical Center
Jackson, MS  39216




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