PBMC volumes via Z1 Particle Counter

Howard Shapiro hms at shapirolab.com
Wed Jul 11 20:34:39 EST 2001


Steve Gaffin wrote-

>         I am trying to establish the best Lower Volume Gate in my Coulter Z1
>for determining the concentration of isolated human PBMCs.
>
>         Coulter cannot provide a value.
>
>         My preliminary correlations with hand counting suggest  60-65 fl.
>Does anyone have another, better value?

65 fl is less than normal RBC volume.  My recollection is that lymphocytes
are 180-200 fl and granulocytes and monocytes are bigger.

-Howard





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