PBMC volumes via Z1 Particle Counter
hms at shapirolab.com
Wed Jul 11 20:34:39 EST 2001
Steve Gaffin wrote-
> I am trying to establish the best Lower Volume Gate in my Coulter Z1
>for determining the concentration of isolated human PBMCs.
> Coulter cannot provide a value.
> My preliminary correlations with hand counting suggest 60-65 fl.
>Does anyone have another, better value?
65 fl is less than normal RBC volume. My recollection is that lymphocytes
are 180-200 fl and granulocytes and monocytes are bigger.
More information about the Cytometry