Alternative for streptavidin-RED670

Larry Arnold lwarma at med.unc.edu
Tue Jul 10 17:00:47 EST 2001


Arnold

CyChrome from Pharmingen is my preferred source of this
reagent.  Alternatively to use the 633 line try Cy5 or Alexa647 (we have
tried the later and it works well).

Larry

At 05:52 AM 7/10/2001, you wrote:

>Greetings all,
>
>Over the last few years I have been assaying G-CSF receptors using
>biotinylated ligand on various cell types. By far the best second layer for
>maost cell types has been streptavidin-RED670  from Gibco/BRL, sadly due to
>the invitrogen merger, many lines have been discontinued -one of them being
>my second layer of choice.
>
>I wonder if any others have had some experience of alternate second layers;
>the excitation may be either 488 or 633.
>
>
>Regards,
>
>
>Arnold
>
>_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
>         Arnold Richard Pizzey
>         Department of Haematology
>         Royal Free and University College London Medical School
>         98 Chenies Mews
>         London WC1E 6HX
>         U.K
>
>         voice:  +44 020-7679-6234
>         Fax:    +44 020-7679-6222
>         email:  a.pizzey at ucl.ac.uk
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Larry W. Arnold, Ph.D.
Res. Assoc. Prof.
Director, Flow Cytometry Facility
Department of Microbiology and Immunology
Lineberger Comprehensive Cancer Center
CB# 7290
University of North Carolina at Chapel Hill
Chapel Hill, NC 27599
Phone: 919-966-1530
FAX: 919-962-8103




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