Alternative for streptavidin-RED670

Arnold Richard Pizzey a.pizzey at UCL.ac.uk
Tue Jul 10 04:52:26 EST 2001


Greetings all,

Over the last few years I have been assaying G-CSF receptors using
biotinylated ligand on various cell types. By far the best second layer for
maost cell types has been streptavidin-RED670  from Gibco/BRL, sadly due to
the invitrogen merger, many lines have been discontinued -one of them being
my second layer of choice.

I wonder if any others have had some experience of alternate second layers;
the excitation may be either 488 or 633.


Regards,


Arnold

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	Arnold Richard Pizzey
	Department of Haematology
	Royal Free and University College London Medical School
	98 Chenies Mews
	London WC1E 6HX
	U.K

	voice:	+44 020-7679-6234
	Fax:	+44 020-7679-6222
	email:	a.pizzey at ucl.ac.uk
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