Alternative for streptavidin-RED670

Arnold Richard Pizzey a.pizzey at
Tue Jul 10 04:52:26 EST 2001

Greetings all,

Over the last few years I have been assaying G-CSF receptors using
biotinylated ligand on various cell types. By far the best second layer for
maost cell types has been streptavidin-RED670  from Gibco/BRL, sadly due to
the invitrogen merger, many lines have been discontinued -one of them being
my second layer of choice.

I wonder if any others have had some experience of alternate second layers;
the excitation may be either 488 or 633.



	Arnold Richard Pizzey
	Department of Haematology
	Royal Free and University College London Medical School
	98 Chenies Mews
	London WC1E 6HX

	voice:	+44 020-7679-6234
	Fax:	+44 020-7679-6222
	email:	a.pizzey at

More information about the Cytometry mailing list