beth.rees at dhhs.tas.gov.au
Mon Jul 9 02:17:12 EST 2001
Many thanks for the replies re CD30.
It appears that CD30 can stain surface membrane and/or golgi bodies .
On Friday we had a peripheral blood from someone with ?large anaplastic
lymphoma. About 50% of cells were CD45-CD7weak+, and negative for a range
of other lymphoid and myeloid markers, including surface CD30. Having just
received the suggestion from the mailing list about the golgi bodies
(thanks, Ierachmiel), I restained the CD30, relying on the FACSlyse and
fixative to permeabilise the already stained cells. Half of the cells
stained positive for intracellular CD30, so it looks like we'll be
incorporating a cytoplasmic CD30 stain into our panel, or alternatively
restaining after lysis and fixation.
many thanks, people, what would I do without you?
Flow Cytometry Lab
Royal Hobart Hospital
ph. 03 6222 8913
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