Yeast Methods

Jan Hesketh j.hesketh at
Tue Jul 3 21:57:04 EST 2001

I have had an enquiry from someone in the brewing industry regarding flow
cytometry methods for yeasts. They are specifically interested in Cell cycle
analysis with PI, Viability with PI/ Fluorescein diacetate, Intracellular
pH, Neutral lipids with Nile Red?, Glycogen with acriflavin, Mitochondria
with Rhodamine 123, flocculation with ConA-FITC lectin.

I have had no experience with any of these areas and was hoping that someone
out there may be able to point me in the right direction. Any tips or
references would be much appreciated.


Jan Hesketh
Molecular Medicine
University of Auckland
phone 09 373 7599 ex 4492
fax   09 373 7492

More information about the Cytometry mailing list