placental digestion

Julie Moore julmoore at calc.vet.uga.edu
Fri Sep 29 12:11:57 EST 2000


Bill--

For human placenta, check out Ritson and Bulmer, J Immun Methods,
1987, 104:231-6.  Be aware, however, that enzymatic dispersal methods
do tend to affect cell phenotypes.  If you are interested in looking
specifically at human intervillous blood, check out the following
refs:
1) Rasheed et al., J Immunol Methods, 1992. 146:185-93.
2) Camelo et al., Fetal Diagn Ther, 1995. 10:322-325.
3) Moore et al, J Immunol Methods, 1997. 209, 93-104.

We use the latter routinely.  It's very gentle and yields a huge
amount of maternal intervillous blood cells that are free from fetal
cell contamination.

Good luck!

Julie Moore

Date:          Wed, 27 Sep 2000 13:49:27 -0700
To: cyto-inbox
From:          Bill Hyun <hyun at cc.ucsf.edu>
Subject:       placental digestion


Hello,
Does anyone have a protocol or reference for digesting mouse or human
placenta to single cells for sorting?
Thanks.
-Bill
--
*****************************
William Hyun, Director
Laboratory for Cell Analysis
UCSF Cancer Center, Room S129
San Francisco, CA  94143-0808

Office (415)476-2632
Lab (415)476-2631
FAX (415)476-8218
email hyun at cc.ucsf.edu

Deliveries:
2340 Sutter Street, Room S129
San Francisco, CA 94115

Julie Moore, PhD
Center for Tropical and Emerging Global Diseases
AND Department of Medical Microbiology and Parasitology
College of Veterinary Medicine
University of Georgia
office: 706-542-5789
lab: 706-542-6758
fax: 706-542-0059
email: julmoore at calc.vet.uga.edu



More information about the Cytometry mailing list