IL-10 detection

Lynn Kord lynn_kord at hotmail.com
Fri Sep 29 12:47:13 EST 2000


Dear fellow flowers...
I am trying to detect IL-10 intracellularly in melanoma tumor cells and
PBL's. I am looking for permeabilization reagents and amplification of
signal recommendations. The tenative scenario is-- mouse anti human IL-10
with goat anti mouse-PE. Is there a better way to amplify signal?
Also, I do not want to use any golgi-stop reagents to increase the IL-10.
Thank you for any recommendations...
Lynn Kord
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