Sperm testing in FACSCalibur

Yenchi_Chen@bd.com Yenchi_Chen at bd.com
Wed Sep 27 22:03:43 EST 2000

Dear Flowers:
I am interested in sperm testing in FACSCalibur. We use rhodamine 123 and PI to
test rat sperm cells. However, all the cells are stained both dyes. The
incubation temperature is 37 centigrade degrees. Low flow rate is set to deliver
the sample to the flow cell. We would like to seeking help from those expertise
at sperm flow analysis. We really appreciate if you can give us some suggestions
and comments on what else we need to take into consideration before we analyse
sperms in Calibur?
Thank you very much.

Yen-Chi Chen, M.S.
CAD Application Specialist
BD Worldwide Inc., Taiwan Branch
Taipei, Taiwan, R.O.C.

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