Lysing solution

Philippe Bourin bourin at attglobal.net
Wed Sep 20 10:46:11 EST 2000


You could do it your self, I think the formula is on the bottle.

"Houston, Jim" wrote :

> To All:
>
> Our lab is using a lysing solution produced by Ortho.  It comes in a small
> bottle and makes 100ml of solution.
> Ortho has discontinued the item.  The product in the bottle is the standard
> Ammonium Chloride reagent.  It is handy in the clinical environment in that
> it is easily prepared and comes with the proper QC/Paperwork.
>
> I am looking for a substitute.
>
> Any ideas besides making it up from scratch?  Is there a like product on the
> market? No fixative.
>
> All help will be appreciated.
>
> Jim Houston
> Coordinator, Flow Cyometry and Cell Sorting
> Cell and Gene Therapy, BMT-RES
> St. Jude Children's Research Hospital
> Memphis, TN 38105
>
> Ph:901-495-2926 or x4155
> E-mail: jim.houston at stjdue.org




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