labelling of Chlamydia bacteria

Dennis J. Young djyoung at UCSD.Edu
Tue Sep 19 13:26:49 EST 2000


Which of the DOZENS of GFP's do you mean ;) ?

Wild type 31,079 daltons
S65T      31,093 daltons
(in theory)

 Andreas Thiel wrote:
>
>Hi flowers,
>
>does anyone have experience in permanent fluorescent labelling of live
>bacteria, in particular Chlamydia trachomatis ? We would like to quantify
>infection rates in monocytes.
>
>Any help would greatly be appreciated.
>
>Thanks in advance!
>
>
>Andreas Thiel
>
>
>Dr. Andreas Thiel
>Clinical Immunology
>Schumannstr. 21/22
>10117 Berlin
>Germany
>
>phone    ++49 30 28460 682
>
>
>email:thiel at drfz.de
>
>
---Dennis

Dennis J. Young
Flow Cytometry Core Facility
University of California, San Diego
Internal Medicine Group, Bldg #4, Rm 126
9500 Gilman Drive
La Jolla  CA  92093-0671

Mail:<<mailto:djyoung at ucsd.edu>>
WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>>
Telephone:(858) 822-0407	FAX: (858) 822-0403



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