proliferative responses

Mattapallil jjmattapallil at UCDAVIS.EDU
Tue Sep 19 18:20:31 EST 2000


Jose hi

Have you tried using the Brdu incorporation assay. It seems to work
well and give better results. You can either do flow or regular color
enhancement with it (read with a regular ELISA plate reader).

Joseph Mattapallil, B.V.Sc., Ph.D.


At 12:25 PM -0700 9/19/00, Jose Benito wrote:
>Hi everyone, I am trying to set up a colorimetric assay to measure
>lymphoproliferative responses to mitogens and recall antigens. I have tried
>the Alamar Blue without any sucess. I will probably try also the celltiter
>assay from promega. Does anyone have experience with this kind of assays?
>Can they really substitute the 3Hthymidine assay?
>Thanks
>Jose Miguel
>Hospital Carlos III
>Madrid
>Spain
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