FW: Research Triangle Cytometry Association 26-Sep-2000

Witherspoon, Sam sw11527 at glaxowellcome.com
Mon Sep 18 18:57:42 EST 2000


> Research Triangle Cytometry Association
> Meeting Announcement
>
>
> "High resolution immunophenotyping of lymphocytic cells by enzymatic
> amplification staining (EAS)"
>
> David Kaplan, M.D./Ph.D.
> Professor of Pathology
> Case Western Reserve University
> Cleveland, Ohio
>
> Abstract:  Flow cytometric analysis is a powerful technique for the single
> cell
> assessment of cell surface expression of selected molecules.  The major
> deficiency of flow cytometry has been its relative insensitivity.  Only
> molecules expressed in abundance have been readily observed.
> Consequently,
> we have developed an enzymatic amplification procedure for the analysis of
> cell surface molecules by flow cytometry.  Transformed and nontransformed
> cells expressing MHC class I, CD5, CD3, CD4, CD6, CD7, CD34, CD45, MHC
> class II, Fas ligand, and phosphatidylserine were assessed.  Our enzymatic
> amplification technology increased the fluorescence signal between 10 and
> 100 fold for all surface molecules tested.  Enzymatic amplification
> staining (EAS) produces a significant enhancement in the resolving power
> of
> flow cytometric analysis of cell surface molecules.  Using this technique
> we have been able to detect the presence of molecules that could not be
> observed by the standard procedure.
>
> Tuesday, September 26, 2000
> 9:00 AM
>
> Duke North Hospital
> Lecture Hall 2001
> Durham, NC
>
> Product information from Flow-Amp Systems will be available after the
> presentation.
>
> Park in the deck on Erwin Road across from Duke North Hospital.  Walk
> across the street through the main entrance to the hospital and take the
> elevators to the 2nd floor.  Proceed straight toward the front entrance on
> the elevated walkway to Room 2001.
>
> We gratefully acknowledge Duke University Comprehensive Cancer Center for
> reimbursing the expenses of this meeting.  For more information contact
> Mike Cook (919) 613-7819 or cook0016 at mc.duke.edu.
>
>	Cheers,
>		Sam
> Sam Witherspoon
> sw11527 at glaxowellcome.com
>
> Dept. of Receptor Biochemistry  Tel. 919-483-3078
> Glaxo Wellcome R&D		  Page 919-857-7768
> 5 Moore Dr.			  Fax  919-483-0585
> RTP, NC  27709
>




More information about the Cytometry mailing list