FW: Research Triangle Cytometry Association 26-Sep-2000
sw11527 at glaxowellcome.com
Mon Sep 18 18:57:42 EST 2000
> Research Triangle Cytometry Association
> Meeting Announcement
> "High resolution immunophenotyping of lymphocytic cells by enzymatic
> amplification staining (EAS)"
> David Kaplan, M.D./Ph.D.
> Professor of Pathology
> Case Western Reserve University
> Cleveland, Ohio
> Abstract: Flow cytometric analysis is a powerful technique for the single
> assessment of cell surface expression of selected molecules. The major
> deficiency of flow cytometry has been its relative insensitivity. Only
> molecules expressed in abundance have been readily observed.
> we have developed an enzymatic amplification procedure for the analysis of
> cell surface molecules by flow cytometry. Transformed and nontransformed
> cells expressing MHC class I, CD5, CD3, CD4, CD6, CD7, CD34, CD45, MHC
> class II, Fas ligand, and phosphatidylserine were assessed. Our enzymatic
> amplification technology increased the fluorescence signal between 10 and
> 100 fold for all surface molecules tested. Enzymatic amplification
> staining (EAS) produces a significant enhancement in the resolving power
> flow cytometric analysis of cell surface molecules. Using this technique
> we have been able to detect the presence of molecules that could not be
> observed by the standard procedure.
> Tuesday, September 26, 2000
> 9:00 AM
> Duke North Hospital
> Lecture Hall 2001
> Durham, NC
> Product information from Flow-Amp Systems will be available after the
> Park in the deck on Erwin Road across from Duke North Hospital. Walk
> across the street through the main entrance to the hospital and take the
> elevators to the 2nd floor. Proceed straight toward the front entrance on
> the elevated walkway to Room 2001.
> We gratefully acknowledge Duke University Comprehensive Cancer Center for
> reimbursing the expenses of this meeting. For more information contact
> Mike Cook (919) 613-7819 or cook0016 at mc.duke.edu.
> Sam Witherspoon
> sw11527 at glaxowellcome.com
> Dept. of Receptor Biochemistry Tel. 919-483-3078
> Glaxo Wellcome R&D Page 919-857-7768
> 5 Moore Dr. Fax 919-483-0585
> RTP, NC 27709
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