Rho 123 and Mitochondrial Transmembrane Potential

Luis Fernando Marques-Santos marques-santos at olimpo.com.br
Wed Sep 13 18:39:08 EST 2000


Dear Flowers,

Is it safe to use Rho 123 to estimate the mitochondrial transmembrane
potential?

If yes,  what is the ideal Rho 123 concentration? I have found a large
difference on the literature (from 5uM to 50nM).

Can I use CCCP (10uM) and Nigericin (10uM) as control?

Thank you all in advance.

Luis Fernando Marques-Santos



¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬
Luis Fernando Marques-Santos
Laboratório de Imunologia Tumoral
Dept. de Bioquímica Médica
CCS - ICB - UFRJ - 21941-590
Rio de Janeiro - Brazil
e-mail: marques-santos at olimpo.com.br
fax: 55 21 270 8647
tel: 55 21 560 88 95 ext. 151
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