Jens Fleischer jfleischer at
Sat Sep 9 07:55:31 EST 2000

The BDIS anti-cytokine antibodies work with the BD Lysing Solution and the
BD Perm Solution. Even if you use PBMC and not whole blood you have to use
the Lysing solution to fix the cells before staining. In contrast, some of
the BD Pharmingen antibodies require their Pharmingen Fix and Perm
Solutions. The recognized epitopes change depending on the method of
fixation and permeabilization. I don't know which "homebrew" recipe may be
compatible with all those clones.


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