Aerosol containment

Joseph M. Beckham joseph.beckham at utsouthwestern.edu
Thu Sep 7 19:25:26 EST 2000


Hi Rich,

I have used the same module on a facstar+ in a BL3 facility sorting HIV
infected cells. Basically, the module creates a slightly negative air
pressure region within the sort chamber to avert the diffusion of small
inhale-able particles into the ambient air. Other than the added noise and
heat of the vacuum pump, I experienced no interference with the over-all use
of the cytometer (sorting, alignment, drop delay, yield, etc). However, the
time and effort spent confirming aerosol containment with T4-phage and E.
coli is another story.

Good luck with your new toy,

~jmb~

on 9/6/00 3:05 PM, Hastings Richard RC at richard.hastings at astrazeneca.com
wrote:

>
> Hi,
>
> I run a Facs Vantage SE. The machine has been great, sorts are pure, number
> of cells sorted is right on the money and drop delay profiles are perfect.
> Before I came to this facility, my boss ordered the aerosol containment
> module for this instrument because he may require the sorting of lentivirus
> infected cells. I have no experience with this piece of hardware. Will this
> get in my way on a day to day basis or does it have a fairly benign
> presence?
>
>
> Thanks, Rich Hastings
>
>
>
>
>
>
>
>
>
>
> Richard C. Hastings
> AstraZeneca Pharmaceuticals
> 1800 Concord Pike
> Wilmington, DE 19803
> (302) 886-2452
> richard.hastings at astrazeneca.com
>




More information about the Cytometry mailing list