Isolation of B cells from whole blood

Louise Affleck l.affleck at adpro.co.uk
Thu Sep 7 04:25:18 EST 2000


Hello again

Thankyou for all your suggestions, I have various protocols to try now...

Thanks again,

Louise

Dr. Louise Affleck
Senior Scientist
Protein Function Group
AdProTech Ltd
Unit 3, 2 Orchard Road
Royston
Herts
SG8 5HD
Tel: +44 (01763) 226090
Fax: +44 (01763) 226021
http://www.adprotech.co.uk
----- Original Message -----
From: "Louise Affleck" <l.affleck at adpro.co.uk>
To: cyto-inbox
Sent: Thursday, August 31, 2000 11:40 AM
Subject: Isolation of B cells from whole blood


>
> Hello fellow flowers,
>
> I don't have access to a sorter or a MACS but I need to isolate B cells
> (ideally CD21+) from, rabbit, marmoset, and rhesus monkey whole blood.
I've
> thought about coupling human C3d (the ligand to CD21) to sepharose then
> passing either buffy coat or lysed blood prep, washing the unbound CD21-
> cells and eluting the CD21+ cells... Any other suggestions would be
> appreciated. If MACS or sorting is the only other possible way then I
could
> probably get access to these pieces of kit....any suggested protocols
would
> be greatly appreciated..
>
> Thanks,
>
> Louise
>
> Dr. Louise Affleck
> Senior Scientist
> Protein Function Group
> AdProTech Ltd
> Unit 3, 2 Orchard Road
> Royston
> Herts
> SG8 5HD
> Tel: +44 (01763) 226090
> Fax: +44 (01763) 226021
> http://www.adprotech.co.uk
>
>




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