Intracellular Cytokine Staining

Joanne Thomas thomas at tritechinc.com
Wed Sep 6 16:12:18 EST 2000


Hi Bernie!
Pharmingen sells positive control cells called "Mick cells" (Mouse
Intracellular Cytokine) which are mouse lymphoid cells which have been
stimulated, fixed and non-permeabilized and express easily detectable levels
of various cytokines. I believe they come in several flavors depending on
the cytokine of interest. MICK-1 would work for gamma IF (cat. no. 20131Z)
and MICK-2 (cat. no. 20132Z) for IL-4.
Joanne


Joanne Thomas, M.S.
Director of Operations
TRITECH Field Engineering
2014 Renard Court, Suite I
Annapolis, MD  21401
1-800-886-7004 (USA)
1-410-266-1522
410-266-0935 (FAX)
thomas at tritechinc.com
-----Original Message-----
From: bernie <bellette at postoffice.utas.edu.au>
To: cyto-inbox
Date: Wednesday, September 06, 2000 4:49 PM
Subject: Intracellular Cytokine Staining


>
>Work in my department involves determining the intracellular cytokine
>stores of murine CD4 and CD8 cells.  Following staining and
>assessment via flow, results to date have not demonstrated many
>positive  cells, even in cell lines known to be producers of the two
>cytokines we are assaying for, IL-4 and IFN-gamma.  Would anyone have
>any trouble shooting tips or know of any cell lines that are
>producers of these cytokines that come pre-stained for intracellular
>cytokines that we can use as a positive control.
>
>Many thanks
>--
>Bernadette Bellette
>PhD Student
>Discipline of Pathology
>University of Tasmania
>
>(03) 6226 4762 (phone)
>(03) 6226 4833 (fax)
>




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