Fischer, Randy (NIAMS) fischer1 at mail.nih.gov
Wed Sep 6 14:17:40 EST 2000


Try saponin.  We add 10µl of a 3% soluiton to approximately 100µl of cells,
wait a few minutes and then add our intracellular staining reagents.  It
does not appear to work for nuclear antigens.

Randy Fischer
NIH/NIAMS
Building 9
Bethesda, MD 20892
fischer1 at mail.nih.gov
(301) 594-3537



> ----------
> From:		KAUR, INDRESHPAL (JSC-SD)
> Sent:		Wednesday, September 6, 2000 9:37 AM
> To:	Cytometry Mailing List
>
>
> Hello
> Does any one know of a reagent that will permeabilize the cell membrane
> and
> not the nuclear membrane. BD's FACS lyse and pharmingens cytofix-cytoperm
> will permeabilize both.
>
> Indresh   Kaur Ph.D.
> Phone: 281-483-8791
>
>
>



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