Question regarding Bone Marrow

Swiggett, Jeanene JSwiggett at carolinas.org
Wed Sep 6 08:32:30 EST 2000


In my past experience assisting physicians collecting and processing bone
marrows I can say that the use of too much heparin in a sample will cause it
to become jelly-like.  With most specimen we used the 5/10ml vials of
injectable heparin that were used in the hospital to flush patients lines.
We would draw up enough heparin into the desired size syringe to allow us to
bring the plunger back and just wet the inside surface of the syringe (~
500ul in a 10ml syringe - but this part is relative) and then expel all
heparin except what was left in the hub and we never had problems.  If you
have any say in how the specimens get drawn I hope this helps.

Jeanene Swiggett, BS MT(ASCP)SH
General Surgery Research
Cannon Research Center
Carolinas Medical Center
Charlotte, NC
704-355-7269
jswigget at carolinas.org



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