visible Ca dyes

Flow Cytometry Facility system at flocyt.int-med.uiowa.edu
Wed Sep 6 08:45:41 EST 2000


Greetings,

We used a combination of calcium green and fura red for calcium flux
studies with a FACScan on Jurkat and human PBL with good success.  We used
FlowJo to produce the ratio of the two fluorochromes.  The changes between
resting state and maximal flux were very similar to indo-1.





At 04:01 PM 09/01/2000 -0400, you wrote:
>
>	A number of years ago we used Fluro-3 red and Snarf-1 in order to use a
>488 laser to measure Ca.  Since then I know  (or think I know) that I have
>seen other dyes used in this manner.
>	I would like to hear from others who have used other visible dyes for
>measuring Ca.
>	Thanks in advance for any and all input.
>	Joan K
>
>
>	Flow Cytometry Lab Supervisor
>	VMRCVM
>	(540) 231-4115
>	FAX 540-231-7367
>	jkalnits at vt.edu
>
>	"It is better to serve than to receive."
>	   B. Borg
>
>
------------------------------------------------
| Justin Fishbaugh			       |
| University of Iowa			       |
| Flow Cytometry Facility		       |
| 48 EMRB				       |
| Iowa City, IA  52242			       |
|					       |
| justin-fishbaugh at uiowa.edu		       |
| http://www.medicine.uiowa.edu/flowcytometry/ |
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