CV question

Zucker.Robert@EPAMAIL.EPA.GOV Zucker.Robert at EPAMAIL.EPA.GOV
Tue Sep 5 15:39:41 EST 2000




Question
There is an effect that has been observed regarding the CV of 2.5 micron
calibration beads using on a flow cytometer. When the peak channel is located at
lower channel numbers the CV is increased. i.e. the CV of a population is higher
at mean channel 100 ( 2.41)  than at mean channel 800 ( 1.25). What is the
statistical explanation for this phenomenon. Does it have to do with less
channels being occupied when the mean is at lower numbers yeilding a higher CV?
Bob


Robert M. Zucker, PhD
U.S. Environmental Protection Agency
MD 72
National Health and Environmental Effects Research Laboratory
Research Triangle Park, North Carolina, 27711
Tel: 919-541-1585; fax 919-541-4017
e-mail: zucker.robert at epa.gov





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